ETD Workshop Seminar Series
Manual Interpretation of Electron Transfer Dissociation (ETD) Mass Spectra of Peptides
Presented by Donald F. Hunt, Departments of Chemistry and Pathology, University of Virginia
Join us for the ABRF Workshop Seminar Series, Manual Interpretation of Electron Transfer Dissociation (ETD) Mass Spectra of Peptides, developed and presented by Donald F. Hunt, Departments of Chemistry and Pathology, University of Virginia.
ABRF 2011 Meeting attendees are welcome to participate in the seminar series. While the seminar is offered at no additional cost, all participants must be registered for the full ABRF meeting. To register, please visit us here.
Workshop Description:
Electron transfer dissociation mass spectrometry is a break-though technology for sequencing post-translationally modified peptides. In this technique, radical anions of polyaromatic hydrocarbons (fluoranthene and azulene) are employed to transfer electrons to the carbonyl group in the polyamide backbone of multiply charged peptides generated by electrospray ionization. Capture of an electron into the peptide backbone reduces the positive charge on the ion by one, and forms a carbonyl radical anion that then abstracts a proton from a nearby protonated amino group. The resulting carbonyl radical triggers cleavage of the adjacent nitrogen-carbon bond to produce fragments of type c’ and z•.
The purpose of the workshop is to provide instruction on how to manually interpret peptide ETD mass spectra. Following a lengthy tutorial about ion structures, fragmentation pathways, predictable changes in fragment ion isotope patterns, etc., we will outline a general approach for the manual interpretation of peptide ETD spectra, solve the sequence of several post-translationally modified peptides, assign homework spectra, and reconvene on the second day of the workshop to go over the homework problems. A packet of lecture notes and handouts will be provided. Attendees should bring an inexpensive calculator, pad of paper, and a small ruler.
Workshop Schedule:
ETD Workshop (Part I) - Monday, February 21, 8:00pm - 11:00pm
ETD Workshop (Part II) - Tuesday, February 22, 3:00pm - 4:15pm & February 22, 4:45pm - 6:00pm (note Part II is a continuation of Part I; attendees should attend both workshops)